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Question 20 for biotechprinciples-statements

Evaluate the process of selecting recombinants based on Antibiotic Resistance.
a. When selecting recombinants using pBR322, one must plate the transformants on two different plates simultaneously.
b. The non-recombinants will lose their antibiotic resistance gene due to the insertion of foreign DNA.
c. The antibiotic resistance gene that remains active helps in selecting the transformants.
d. The antibiotic resistance gene that is inactivated by insertion helps in selecting the recombinants.
e. The cumbersome procedure of double plating has been replaced by methods based on color production using beta-galactosidase.